Peptide Based Hepatitis C Vaccine Design From RNA-dependent RNA polymerase (RdRp) NS5B: Immunoinformatics Approach

Manalu, Rosario Trijuliamos and Setyaningsih, Erwi Putri (2023) Peptide Based Hepatitis C Vaccine Design From RNA-dependent RNA polymerase (RdRp) NS5B: Immunoinformatics Approach. Journal of Research in Pharmaceutical Science, 9 (3). pp. 31-39. ISSN 2347-2995

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Abstract

Hepatitis is an inflammatory disease of the liver that can be caused by various factors. A common cause of hepatitis is a viral infection. Until now, there is no effective vaccine for Hepatitis C Virus infection so it is still a health problem in the world. This study aims to obtain a peptide-based vaccine design that acts as an antigen, does not cause an allergen, is not toxic, and is not homologous to human cells. This study used NS5B RNA-dependent RNA polymerase (RdRp) samples taken from PDB. All stages of the analysis are carried out using the appropriate web server and software. Sequence analysis obtained selected T-cell epitopes, namely LSAFSLHSY, VLDDHYRDV, YLFNWAVKT, TLTCYLKASAACRAA, NTLTCYLKASAACRA, and LTCYLKASAACRAAK. Meanwhile, the selected B-cell epitopes were FCVQPEKGGRK and DSTVTENDIRV. Which was given the connectors EAAAK, AAY, GPGPG, and KK on each of the corresponding epitope connectors. Then, the vaccine candidate is docked and visualized in 2D and 3D. The research shows that the design of the hepatitis C vaccine using the NS5B protein is stable and not homologous to human cells so it can be used as a vaccine candidate.

Item Type:	Article
Subjects:	R Medicine > R Medicine (General)
Depositing User:	Rosario Trijuliamos Manalu
Date Deposited:	14 Apr 2023 02:57
Last Modified:	14 Apr 2023 02:57
URI:	http://repository.istn.ac.id/id/eprint/6215

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